HALAL STUDIES AND PREDICTION OF SULFORAPHANE FROM CABBAGE (BRASICA OLERACEA VAR. CAPITATA L.) AS GLUTATHIONE S-TRANSFERASE (GTS) SUBSTRATE

Abstract

Sulforaphane, a bioactive compound found in cruciferous vegetables, has been widely studied for its potential role in enzymatic detoxification processes, particularly as a substrate for Glutathione S-transferase (GST). GSTs are essential enzymes that facilitate the conjugation of glutathione (GSH) to electrophilic compounds, thereby enhancing their elimination from the body and reducing oxidative stress. In this study, computational analysis was conducted to predict the interaction of sulforaphane with GST. The probability of activity (Pa) was found to be 0.936, indicating a high likelihood that sulforaphane serves as a GST substrate, while the probability of inactivity (Pi) was 0.002, suggesting negligible chances of non-interaction. These findings align with previous studies that demonstrate sulforaphane’s role in inducing GST expression and enhancing detoxification pathways. The strong affinity of sulforaphane for GST enzymes suggests its potential applications in pharmacology, particularly in disease prevention strategies against oxidative stress and toxicant exposure. Moreover, the ability of sulforaphane to modulate GST activity highlights its significance in cancer chemoprevention and cellular defense mechanisms. Future research, including in vitro and in vivo validation, is necessary to further elucidate the kinetics and structural interactions of sulforaphane with GST. The integration of computational predictions with experimental studies will contribute to the development of targeted therapeutic interventions, optimizing the pharmacological benefits of sulforaphane in detoxification and disease prevention.